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Taylor TW-357 - page 1
TW-357 REV E 1 LABS (Laboratory Archival Biological Storage) CRYOSTORAGE SYSTEM OPERATING AND MAINTENANCE INSTRUCTION REVIEW AND UNDERSTAND ALL SAFETY PROCEDURES IN FORM # TW-10 P/ N 7950-8052 BEFORE ATTEMPTING TO INSTALL, OPERAT E OR PERFORM MAINTENANCE ON THIS CRYOSTORAGE SYSTEM. DO NOT ATTEMPT TO USE OR MAINTAIN THIS UNIT UNTIL YOU READ AND UNDE ...
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Taylor TW-357 - page 2
TW-357 REV E 2 ...
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Taylor TW-357 - page 3
TW-357 REV E 3 Table of Contents WARNING 4 Safety Precautions for Liquid Nitrogen 4 GENERAL INFORMATION 6 Text Format Notation 6 LABS Cryostorage Specifications 7 INSTALLATION 9 Unpacking and Inspection 9 Liquid Nitrogen Supply Connection 9 Power Supply Connection 10 KRYOS Control Field Installation 10 OPERATION 11 Initial Fill 11 Control Component ...
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Taylor TW-357 - page 4
TW-357 REV E 4 WARNING The following safety precautions are for your protection. Before installing, operating, or maintaining this unit read and follow all safety precautions in this section a nd in reference publications. Failure to observe all safety precautions can result in property damage, personal injury, or possibly death. Safety Precautions ...
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Taylor TW-357 - page 5
TW-357 REV E 5 Dispose of Waste Liquid Nitrogen Safely Dispose of waste liquid nitrogen out-of-doors where its cold temperature cannot damage floors or driveways and where it will evaporate rapidly. An outdoor pit filled with clean sand or gravel will evaporate liquid nitrogen safely and quickly. Electrical Electrical Shock Can Kill – the liquid ...
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Taylor TW-357 - page 6
TW-357 REV E 6 GENERAL INFORMATION Thank you for purchasing this product. This state of the art CRYOSTORAGE SYSTEM can control either the liquid level and/or the vapor temperature range. The f eatures are designed to provide a safe environment for samples while at the same time tracking all relevant in formation associated with the freezer. This co ...
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Taylor TW-357 - page 7
TW-357 REV E 7 LABS Cryostorage Specifications Liquid nitrogen at atmospheric pressure weighs 1. 78 lb./liter (0.8 kg/liter). All Taylor-Wharton Gas Equipment cryostorage systems are designed to support the full weight of liquid nitrogen and a complete stainless steel or aluminum inventory control system with boxes and specimens. Dimensions 80K 40K ...
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Taylor TW-357 - page 8
TW-357 REV E 8 KRYOS Specifications Configurations: Designed exclusivel y for all Taylor-Wharton LABS CryoStorage Systems Power Supply: 16.5 VAC, 40 Voltamps – Standard Battery Backup Version 24 VAC, 40 Voltamps (No Battery) (For power outlets other than North American 110 VAC contact Taylor-Wharton Customer Service.) Sensor Assembly: Freeze-Guar ...
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Taylor TW-357 - page 9
TW-357 REV E 9 INSTALLATION Unpacking and Inspection Inspect shipping containers for external damage. All claims for dam age (apparent or concealed) or partial loss of shipment must be m ade in writing within five (5) days from receipt of goods. If damage or loss is apparent, please notify the shipping agent immediately. Open the shipping container ...
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Taylor TW-357 - page 10
TW-357 REV E 10 Power Supply Connection Connect the 16.5 Volt AC power supply to the control of the LABS cryostorage sy stem; then plug the power supply into a surge protected 110/120 VAC outlet. KRYOS Control Field Installation 1. Unplug power from old unit. 2. Close liquid nitrogen supply at valve. 3. Remove 4 Phillips head screws from rear of in ...
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Taylor TW-357 - page 11
TW-357 REV E 11 OPERATION These instructions are for operators experienced with cryogenic equipment. Before operating the system, become familiar with the safety precautions in this ma nual and in reference publica tions. Make certain all applicable provisions set forth in the Installation S ection have been followed before placing a system in oper ...
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Taylor TW-357 - page 12
TW-357 REV E 12 Control Components Interface Panel The KRYOS Interface panel, which the user will inter act with, contains the vacuum fluorescent display as well as the number keypad, power button, help button and the soft-key control buttons. (See figure 1.0 below.) Figure 1.0 KRYOS Interface Panel Main Control The “brain” for the control syst ...
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Taylor TW-357 - page 13
TW-357 REV E 13 Lid Switch Is attached to the hinge and determines whether or not the lid is open on the freezer. This also allows the control to determine whether to activate the Qu ickchill, Auto Defog or Lid Alarm features. Lid Switch Figure 3.0 Lid Switch Solenoid Valve Designed to work with 12 VDC solenoid valves manufactur ed by Valcor, Parke ...
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Taylor TW-357 - page 14
TW-357 REV E 14 Operating Parameters When materials are imm ersed in liquid nitrogen, th ey will assume the temperature of the liquid -196ºC (- 320ºF). When material is stored in the vapor phase ove r the liquid, the liquid nitrogen is still a very cold refrigerant, but the refrigerator’s interior temperatur e increases somewhat as product is s ...
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Taylor TW-357 - page 15
TW-357 REV E 15 Thermocouple Positioning The thermocouple is a separate sensor u sed to monitor and control the temper ature within the refrigerator. The factory-supplied thermocouple in the LABS cryostorage system is positioned to monitor the temperature 30” above the tray. Adding an Inventory Control System The purpose of the inventory control ...
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Taylor TW-357 - page 16
TW-357 REV E 16 Inventory Control System Layouts (LABS Cryostorage System) Typical 13-2 Rack 100 Vial Polycarbonate 100 Vial Aluminum Boxes 81 Vial Polycarbonate, Cardboard, or Stainless Steel Boxes LABS-20K NOTE: RACKS ARE NOT SQUARE. ORIENTATION IS IMPORTANT. LABS-80K LABS-40K 2ml vial storage – ask about other Box types Part Number Racks 13 x ...
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Taylor TW-357 - page 17
TW-357 REV E 17 Blood Bag Cassette Blood Bag Storage in Cassette LABS-20K L A B S - 8 0 K L A B S - 4 0 K Fenwal 4R9951 Blood Bags – ask about other Bag types PART NUMBER CAPACITY FRAMES CASSETTES LABS-80K 2100 300 2100 LABS-40K 1204 172 1204 LABS-20K 560 80 560 Figure 6.0 Fenwal 4R59951 Bl ood Bag Rack Arrangements ...
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Taylor TW-357 - page 18
TW-357 REV E 18 Blood Bag Cassette Blood Bag Storage in Cassette LABS-20K LABS-80K LABS-40K Fenwal 4R9953 Blood Bags – ask about other Bag types PART NUMBER CAPACITY FRAMES CASSETTES LABS-80K 1505 301 1505 LABS-40K 820 164 820 LABS-20K 400 80 400 Figure 7.0 Fenwal 4R59953 Blood Bag Rack Arrangements ...
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Taylor TW-357 - page 19
TW-357 REV E 19 Blood Bag Cassette Blood Bag Storage in Cassette LABS-20K LABS-80K LABS-40K Fenwal 4R5462 – 9955 Blood Bags – ask about other Bag types PART NUMBER CAPACITY FRAMES CASSETTES LABS-80K 1050 210 1050 LABS-40K 590 118 590 LABS-20K 290 58 290 Figure 8.0 Fenwal 4R5462 – 9955 Blood Bag Rack Arrangements ...
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Taylor TW-357 - page 20
TW-357 REV E 20 Control Operation This section of the operating manual is for Ta ylor-W harton approved equipment that uses the KRYOS controller. Introduction The KRYOS temperature and LN2 level controller is designed for easy operation and uninterrupted, reliable service. This controller will maintain the selected tem perature and liquid level ran ...
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Taylor TW-357 - page 21
TW-357 REV E 21 Main Display Screen The main display screen consists of 4 lines of information. Line 1: Displays the current status of the control. It indicates if all systems are normal or if any errors have been detected. Error messages disappear when the error is corrected. Line 2: Displays the level sensing in the control. If the 7-thermistor o ...
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Taylor TW-357 - page 22
TW-357 REV E 22 Table 1.0 Menu System 1. Temperature 1.1 Thermocouple Select 1.2 Calibrate Temperature 1.3 Test Temperature System 2. Level Sensing 2.1 Test Level Sensors 2.2 Set Sensor Offset 2.3 Sensor Positions 2.3.1 Start Set Point 2.3.2 Stop Set Point 2.4 Sensor Type 2.5 Inch/Metric 3. Alarms 3.1 High-Temp Alarm #1 3.2 High-Temp Alarm #2 3.3 S ...
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Taylor TW-357 - page 23
TW-357 REV E 23 Help Screens The Help button provides help to the user at any point in the menu system. The help message is displayed and the user is then prompted to press a button to return to the menu system. Temperature Thermocouple Select The chamber temperature is monitored with 1 or 2 Type T therm ocouples. The thermocouple is placed in the ...
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Taylor TW-357 - page 24
TW-357 REV E 24 Sensor Type The sensor type can be set through the menu system ( MENU , 2, 4). The sensor type selection should match the sensor type that is being used in the system. This is a 4-sensor, a 7-sensor (F reeze-Guard) or an 8-sensor array. If the sensor assembly is unplugged and the main control is still on, the display will indicate t ...
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Taylor TW-357 - page 25
TW-357 REV E 25 System Alarms The Low LN2 Supply Alarm can be set through the menu system ( MENU , 3, 3, 1). This alarm is activated if the solenoid valve is not closed within a designated tim e period after a fill starts. The solenoid valve can be closed either automatically (the LN2 level reaches the STOP FILL sensor) or manually (the stop fill b ...
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Taylor TW-357 - page 26
TW-357 REV E 26 The control consolidates the events in the internal log (combining “start ev ent” / ”conclude event” information to provide one event with duration), however, when the da ta is sent out the serial port no consolidation of data occurs. As an example, a fill would provide one log entry in the internal log of the control, indic ...
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Taylor TW-357 - page 27
TW-357 REV E 27 OPEN THERMOCOUPLE LOGGING is accessible through the menu system of the control ( MENU , 4, 2, 4). This menu choice turns on/off the logging of the thermocouple open alarm. The choices are ENABLE and DISABLE. Records data in the error log. HIGH TEMP #1 LOG is accessible through the menu system of the control ( MENU , 4, 2, 5). This m ...
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Taylor TW-357 - page 28
TW-357 REV E 28 The MENU PASSWORD can be set through the menu system of the control ( MENU , 5, 2). The menu password requires entry of a 4-digit password before allowing access to the menu system to change any control settings. Follow the directions on the display to set a new password or to disable a password. Codes 0000 & 9999 are invalid ch ...
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Taylor TW-357 - page 29
TW-357 REV E 29 Menu Password When the menu password is active, it is possible to re view all of the control settings without having the password. When a password is activated and the u ser presses the menu key, two choices are available: 1) MENU (PASSWORD REQ) 2) CONTROL SETTINGS By selecting choice #2 the user can go through the menu system and s ...
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Taylor TW-357 - page 30
TW-357 REV E 30 Control Range CONTROL RANGE can be accessed through the menu system of the control ( MENU , 6, 2, 3, 2, 2). KRYOS maintains a temperature range around the user-selected te mperature. A tighter range maintains a tem perature very close to the selected temperat ure at the cost of greater LN2 usage. A broad range provides more temperat ...
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Taylor TW-357 - page 31
TW-357 REV E 31 Display Brightness DISPLAY BRIGHTNESS can be set through the menu system of the control ( MENU , 6, 3). This option changes the intensity of the display. The possible choices are 25%, 50%, 75% and 100%. About this Control ABOUT THIS CONTROL can be accessed in the menu system ( MENU , 6, 4). This option provides information about the ...
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Taylor TW-357 - page 32
TW-357 REV E 32 The DC solenoid valve requires that the battery be connected for the sy stem to work properly. If the battery is disconnected or completely discharged the valve opera tion and the control operation may be affected. In particular, if the control is calling for a fill, the sole noid will be called to open. Opening the solenoid takes a ...
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Taylor TW-357 - page 33
TW-357 REV E 33 MAINTENANCE Strainer Cleaning Instructions The container will not fill properly if the strainer is clogge d with ice or dirt. To clean the strainer, first close the supply valve to the refrigerator. Vent the fill line of all pressure. Remove and warm the strainer to am bient temperature. Purge the strainer from both directions with ...
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Taylor TW-357 - page 34
TW-357 REV E 34 Cleaning Your Taylor-Wharton CryoStorage System The cryogenic vessel of all LABS CryoStorage Systems ma y need to be cleaned, and sterilized if the type of stored product is changed or the unit is taken out of service. The vessel must be defrosted, cleaned, and sterilized regardless of the type of stored product, prior to return to ...
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Taylor TW-357 - page 35
TW-357 REV E 35 WARNING: Never use chlorine-based disinfect ants to clean a LABS CryoStorage System. Normal Evaporation Rate (NER) Test Nitrogen consumption is an accumulation of all system components and user introduced evaporation. The storage chamber is a double walled, vacuum insulated vessel and contributes to the daily consumption of liquid n ...
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Taylor TW-357 - page 36
TW-357 REV E 36 The Kryos Control installed on the Taylor-Wharton LABS 80K refrigerator operates w ith software version 9.0 (regular operation) or 9.0B (battery backup). This control software has b een specially designed to match the design characteristics of the LABS 80K refrigerator. Th e differences between the versions are in the table below Ta ...
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Taylor TW-357 - page 37
TW-357 REV E 37 • Move the sensor up or down so that the appropria te numbers of sensors are in liquid (read “L”). • Get back to the KRYOS main screen and the level indi cated should ma tch the physically measured reading. The Sensor Offset, the Start Fill and the Stop Fill can all be set through the KRYOS menu system ( MENU , 2). Removing/ ...
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Taylor TW-357 - page 38
TW-357 REV E 38 Sensor Plug Schematic Figure 9.0 Pin View Schematics ...
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Taylor TW-357 - page 39
TW-357 REV E 39 LABS Plumbing Assembly Solenoid Valve Freeze-Guard Sensor Male Branch Tee 90 deg Elbow 90 deg Elbow Male Branch Tee Reducer Relief Valve Tube Strainer Female Connector Relief Valve Figure 10.0 Plumbing Assembly ...
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Taylor TW-357 - page 40
TW-357 REV E 40 TROUBLESHOOTING Symptoms The key to troubleshooting your LABS Cryo Storage system is to determine which component in the system is the source of the problem. Determine if the problem is occurring in any of the following subsystems: Supply Vessel, Transfer Line, Power Source, Temperature, Le vel Sensing, Security, Lid Switch, Solenoi ...
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Taylor TW-357 - page 41
TW-357 REV E 41 • Sim ulate an over fill by lowering the next thermi stor into the LN2 . HIGH LEVEL alarm should sound with 10 seconds. • Re-install sensor assem bly and thermocouple into sen sor tube as before. Open supply valve on LN2 supply. Indicates Low LN2 Supply When the START FILL thermistor is uncovered, the cont roller calls for the f ...
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Taylor TW-357 - page 42
TW-357 REV E 42 Fill Solenoid Cycles On and Off KRYOS features a Freeze-Guard function whereby the valv e open duration is limited, allowed to shut for a short warm-up and then opened again. To c onfirm or change the valve open duration: • Select MENU , USER OPTIONS, CONTROL OPTIONS, FREEZE-GUARD OPTIONS, VALVE OPEN DURATION. If the controller de ...
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Taylor TW-357 - page 43
TW-357 REV E 43 Defog Feature Will Not Operate See LID OPEN ALARM. To activate or adjust times: Select MENU , USER OPTIONS, CONTROL OPTIONS, LID/DEFOG SETTINGS, AUTO DE-FOG. Push Buttons Will Not Respond 1. Check all ribbon cable connectors on back of display panel. 2. Moisture may have migrated into the sealed faceplate. Call Taylor-Wharton for a ...
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Taylor TW-357 - page 44
TW-357 REV E 44 Factory Settings To restore factory settings at any time KRYOS can be re-initialized. Please note that a re-initialization clears all logs and passwords and resets settings to those listed in the table below. To reset factory settings in the control, go to the menu choice ABOUT THIS CONTROL. ( MENU , 6, 4) and while looking at this ...
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Taylor TW-357 - page 45
45 REPLACEMENT PARTS Refrigerator Parts (LABS) 80K/40K/20K Lid Assembly……………………………………………………………LA80-9C85/LA40-9C85/LA20-9C85 Caster, ……………….. …………………………………………………………… 7300-8040 Decal, Warning………………………………………………… ...
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Taylor TW-357 - page 46
46 Fill in top section at installation. Copy this form each time service is required. Fill bottom section with service notes to keep a complete log of each freezer service and maintenance history. End User compan y Name_________________________________ LOG NO_______________________________ Service Contract Name/Company______________________________ ...
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Taylor TW-357 - page 47
47 4075 Hamilton Blvd. Theodore, Alabama 36582 U.S.A. Telephone (251) 443-8680 Fax (251) 443-2250 In U.S. and Canada: (800) TW TANKS (898-2657) ©2002 Harsco Corporation ...
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